p38 mapk rabbit ab  (Abmart Inc)

Journal: Poultry Science

Article Title: Pathogenic mechanism of Eimeria tenella Et MIC2 promotes Eimeria tenella invasion and inhibits host cell apoptosis through binding to the ITGAV receptor

doi: 10.1016/j.psj.2026.106922

Figure Lengend Snippet: The mRNA expression of ITGAV, FAK, PLC, PKC, p65, ERK, JNK, p38, PI3K, Akt, Bax, Bcl2 , and Caspase 3 in E.tenella host cells.

Article Snippet: Membranes were blocked with 5 % BSA at 37°C for 2 h and incubated overnight at 4°C with the following primary antibodies: ITGAV Rabbit Ab, PLC Rabbit Ab, p-PLC Rabbit Ab, p-p65 Rabbit Ab, and Bcl2 Rabbit Ab were purchased from Bioss (Beijing, China); FAK Rabbit Ab, p-FAK Rabbit Ab, ERK Rabbit Ab, p-ERK Rabbit Ab, JNK Rabbit Ab, p-JNK Rabbit Ab, p38 MAPK Rabbit Ab, p-p38 MAPK Rabbit Ab, PI3K Rabbit Ab, p-PI3K Rabbit Ab, AKT Rabbit Ab, p-AKT Rabbit Ab, PKC Rabbit Ab, p-PKC Rabbit Ab, Bax Rabbit Ab, and Caspase 3 Rabbit Ab were purchased from Abmart (Shanghai, China); p65 Rabbit Ab (Proteintech, Wuhan, China).

Techniques: Expressing

Journal: Poultry Science

Article Title: Pathogenic mechanism of Eimeria tenella Et MIC2 promotes Eimeria tenella invasion and inhibits host cell apoptosis through binding to the ITGAV receptor

doi: 10.1016/j.psj.2026.106922

Figure Lengend Snippet: The protein activity changes of ITGAV, FAK, PLC, PKC, p65, ERK, JNK, p38, PI3K, Akt, Bax, Bcl2, and Caspase 3 in E.tenella host cells.

Article Snippet: Membranes were blocked with 5 % BSA at 37°C for 2 h and incubated overnight at 4°C with the following primary antibodies: ITGAV Rabbit Ab, PLC Rabbit Ab, p-PLC Rabbit Ab, p-p65 Rabbit Ab, and Bcl2 Rabbit Ab were purchased from Bioss (Beijing, China); FAK Rabbit Ab, p-FAK Rabbit Ab, ERK Rabbit Ab, p-ERK Rabbit Ab, JNK Rabbit Ab, p-JNK Rabbit Ab, p38 MAPK Rabbit Ab, p-p38 MAPK Rabbit Ab, PI3K Rabbit Ab, p-PI3K Rabbit Ab, AKT Rabbit Ab, p-AKT Rabbit Ab, PKC Rabbit Ab, p-PKC Rabbit Ab, Bax Rabbit Ab, and Caspase 3 Rabbit Ab were purchased from Abmart (Shanghai, China); p65 Rabbit Ab (Proteintech, Wuhan, China).

Techniques: Activity Assay

Journal: Poultry Science

Article Title: Pathogenic mechanism of Eimeria tenella Et MIC2 promotes Eimeria tenella invasion and inhibits host cell apoptosis through binding to the ITGAV receptor

doi: 10.1016/j.psj.2026.106922

Figure Lengend Snippet: The mRNA expression of ITGAV, FAK, PLC, PKC, p65, ERK, JNK, p38, PI3K, Akt, Bax, Bcl2 , and Caspase 3 in E.tenella host cells.

Article Snippet: p-p38 MAPK Rabbit Ab , Abmart , TA4001 , 1: 1500.

Techniques: Expressing

Journal: Poultry Science

Article Title: Pathogenic mechanism of Eimeria tenella Et MIC2 promotes Eimeria tenella invasion and inhibits host cell apoptosis through binding to the ITGAV receptor

doi: 10.1016/j.psj.2026.106922

Figure Lengend Snippet: The protein activity changes of ITGAV, FAK, PLC, PKC, p65, ERK, JNK, p38, PI3K, Akt, Bax, Bcl2, and Caspase 3 in E.tenella host cells.

Article Snippet: p-p38 MAPK Rabbit Ab , Abmart , TA4001 , 1: 1500.

Techniques: Activity Assay

Journal: Communications Biology

Article Title: Assessing phototoxic drug properties of hydrochlorothiazide using human skin biopsies

doi: 10.1038/s42003-025-08064-1

Figure Lengend Snippet: A Representative Western blots demonstrating phosphorylation of p38 MAPK (T180/Y182) and total p38 MAPK protein in untreated control biopsies (Ctrl) and in biopsies treated with HCT 6 h after irradiation with 300 mJ/cm 2 UVA or UVB. Unirradiated biopsies served as group-specific control (non). B Quantification of phospho-p38 MAPK in relation to total p38 MAPK protein. C Gene expression of pro-inflammatory marker Tumor Necrosis Factor alpha ( TNFα ) and Interleukin 6 ( IL6 ) and ( D ) Connective Tissue Growth Factor ( CTGF ) normalized against Glyceraldehyde 3-Phosphate Dehydrogenase ( GAPDH ) 6 h after irradiation. E Representative Western blots demonstrating phosphorylation of p38 MAPK (T180/Y182) and total p38 MAPK protein in Ctrl, or HCT 24 hours after irradiation with 300 mJ/cm 2 UVA or UVB. Unirradiated biopsies served as group-specific control. F Quantification of phospho-p38 MAPK in relation to total p38 MAPK protein. G Gene expression of pro-inflammatory marker Tumor Necrosis Factor alpha ( TNFα ) and Interleukin 6 (IL6 ) and ( H ) Connective Tissue Growth Factor ( CTGF ) normalized against GAPDH 24 h after irradiation. For ( B, C, D, F, G, H ) n = 6 biopsies per group. Data are shown as mean ± SEM, with individual data points. For comparison of three groups P -value was determined using Kruskal-Wallis with Dunn´s multiple comparisons test for Ctrl group in ( B and F ) and an One-way ANOVA with Tukey multiple comparisons test for HCT group in ( B and F ). For ( C, D, G and H ) an One-way ANOVA with Tukey multiple comparisons test was used. IOD: Integrated optical density. Numerical source data are provided within the Supplementary Data .

Article Snippet: Membranes were blocked in 1xPBS-T (Phosphate-Buffered Saline: 137 mmol/L NaCl; 2.7 mmol/L KCl; 4.3 mmol/L Na 2 HPO 4 ; 1.47 mmol/L KH 2 PO 4 , pH 7.4 containing 0.1% Tween) containing 5% non-fat dry milk for at least 120 min at room temperature and exposed to the following primary antibodies overnight: Anti-γH2A.X (phospho S139), rabbit monoclonal Ab, Abcam, #ab81299, diluted 1:1000), anti-p53 rabbit Ab (#9282S; Cell Signaling Technologies, diluted 1:1000), anti-phospho-p53 (S15) rabbit Ab (#9284S; Cell Signaling Technologies, diluted 1:1000), anti-p38 MAPK rabbit Ab (#9212S; Cell Signaling Technologies, diluted 1:1000), anti-phospho-p38 MAPK (T180/Y182) (12F8) rabbit Ab (#4631S; Cell Signaling Technologies, diluted 1:1000), anti-Catalase rabbit mAb (#14097S, Cell Signaling Technologies, diluted (1:1000), anti-superoxide dismutase 1 (SOD-1) rabbit polyclonal IgG (sc-11407, Santa Cruz Biotechnology, diluted 1:5000), anti-superoxide dismutase 2 (SOD-2) rabbit polyclonal IgG (sc-30080, Santa Cruz Biotechnology, diluted 1:5000), anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH; MAB374, Millipore, Darmstadt, Germany, diluted (1:10000)).

Techniques: Western Blot, Phospho-proteomics, Control, Irradiation, Gene Expression, Marker, Comparison

Journal: Communications Biology

Article Title: Assessing phototoxic drug properties of hydrochlorothiazide using human skin biopsies

doi: 10.1038/s42003-025-08064-1

Figure Lengend Snippet: A Representative Western blots demonstrating protein level of tumor suppressor protein p53, phosphorylation of histone H2A.X (γH2A.X, Serin139) and phosphorylation of p53 (Serin15) in untreated control biopsies (Ctrl), and in biopsies treated with HCT six hours after irradiation with 5 J/cm2 UVA. Unirradiated biopsies served as group-specific control (non). Protein expression of Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) served as loading control. Quantification of ( B ) p53 protein, ( C ) phospho-p53 (Ser15), and ( D ) DNA damage marker γH2A.X. E Gene expression of p53-regulator MDM2 normalized against GAPDH 6 h after irradiation ( F ) Representative double-stained immunohistochemistry for γH2A.X and p53 of human skin biopsies 6 h after irradiation with 5 J/cm2 UVA. G Quantification of p53 positive stained nuclei and ( H ) γH2A.X positive stained nuclei 6 h after UVA irradiation in the epidermis of Ctrl or HCT-treated skin biopsies. I Representative Western blots demonstrating phosphorylation of p38 MAPK (T180/Y182) and total p38 MAPK protein in Ctrl, or HCT 6 hours after irradiation with 5 J/cm2 UVA. Unirradiated biopsies served as group-specific control (non). J Quantification of phospho-p38 MAPK in relation to total p38 MAPK protein. K Gene expression of pro-inflammatory marker Tumor Necrosis Factor alpha ( TNFα ), Interleukin 6 ( IL6 ), Interleukin 1β ( IL1β ), and ( L ) Connective Tissue Growth Factor ( CTGF ) normalized against GAPDH 6 h after irradiation. For ( B, C, D, E, G, H, J, K, L ) n = 3 biopsies per group. Data are shown as mean ± SEM with individual points. For comparison of groups, P -value was determined using Kruskal-Wallis with Dunn´s multiple comparisons test for all groups in ( B, C, D, E, G, H, J, K, L ). A Mann-Whitney test (#) was used for comparison of 2 groups for ( D, H, J ). IOD: Integrated optical density. Numerical source data are provided within the Supplementary Data file.

Article Snippet: Membranes were blocked in 1xPBS-T (Phosphate-Buffered Saline: 137 mmol/L NaCl; 2.7 mmol/L KCl; 4.3 mmol/L Na 2 HPO 4 ; 1.47 mmol/L KH 2 PO 4 , pH 7.4 containing 0.1% Tween) containing 5% non-fat dry milk for at least 120 min at room temperature and exposed to the following primary antibodies overnight: Anti-γH2A.X (phospho S139), rabbit monoclonal Ab, Abcam, #ab81299, diluted 1:1000), anti-p53 rabbit Ab (#9282S; Cell Signaling Technologies, diluted 1:1000), anti-phospho-p53 (S15) rabbit Ab (#9284S; Cell Signaling Technologies, diluted 1:1000), anti-p38 MAPK rabbit Ab (#9212S; Cell Signaling Technologies, diluted 1:1000), anti-phospho-p38 MAPK (T180/Y182) (12F8) rabbit Ab (#4631S; Cell Signaling Technologies, diluted 1:1000), anti-Catalase rabbit mAb (#14097S, Cell Signaling Technologies, diluted (1:1000), anti-superoxide dismutase 1 (SOD-1) rabbit polyclonal IgG (sc-11407, Santa Cruz Biotechnology, diluted 1:5000), anti-superoxide dismutase 2 (SOD-2) rabbit polyclonal IgG (sc-30080, Santa Cruz Biotechnology, diluted 1:5000), anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH; MAB374, Millipore, Darmstadt, Germany, diluted (1:10000)).

Techniques: Western Blot, Phospho-proteomics, Control, Irradiation, Expressing, Marker, Gene Expression, Staining, Immunohistochemistry, Comparison, MANN-WHITNEY

Journal: Communications Biology

Article Title: Assessing phototoxic drug properties of hydrochlorothiazide using human skin biopsies

doi: 10.1038/s42003-025-08064-1

Figure Lengend Snippet: A Representative Western blots demonstrating protein level of tumor suppressor protein p53, phosphorylation of histone H2A.X (γH2A.X, Serin139) and phosphorylation of p53 (Serin15) in untreated control biopsies (Ctrl), and in biopsies treated with HCT 24 h after irradiation with 5 J/cm 2 UVA. Unirradiated biopsies served as group-specific control (non). Protein expression of Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) served as loading control. Quantification of ( B ) p53 protein, ( C ) phospho-p53 (Ser15), and ( D ) DNA damage marker γH2A.X. E Gene expression of p53-regulator MDM2 normalized against GAPDH 24 h after irradiation. F Representative double-stained immunohistochemistry for γH2A.X and p53 of human skin biopsies 24 h after 5 J/cm 2 UVA. G Quantification of p53 positive stained nuclei and ( H ) γH2A.X positive stained nuclei 24 h after UVA irradiation in the epidermis of Ctrl or HCT-treated skin biopsies. I Representative Western blots demonstrating phosphorylation of p38 MAPK (T180/Y182) and total p38 MAPK protein in Ctrl, or HCT 24 h after irradiation with 5 J/cm2 UVA. Unirradiated biopsies served as group-specific control (non). J Quantification of phospho-p38 MAPK in relation to total p38 MAPK protein. K Gene expression of pro-inflammatory marker Tumor Necrosis Factor alpha ( TNFα ), Interleukin 6 ( IL6 ), Interleukin 1β ( IL1β ), and ( L ) Connective Tissue Growth Factor ( CTGF ) normalized against GAPDH 24 h after irradiation. Data are shown as mean ± SEM with individual points. For ( B, C, D, E, G, H, J, K, L ) n = 3 biopsies per group. For comparison of groups P -value was determined using Kruskal-Wallis with Dunn´s multiple comparisons test for ( B, C, D, E, G, H, J, K, L ). A Mann-Whitney test (#) was used for comparison of 2 groups for ( D, H, J ). IOD: Integrated optical density. Numerical source data are provided within the Supplementary Data file.

Article Snippet: Membranes were blocked in 1xPBS-T (Phosphate-Buffered Saline: 137 mmol/L NaCl; 2.7 mmol/L KCl; 4.3 mmol/L Na 2 HPO 4 ; 1.47 mmol/L KH 2 PO 4 , pH 7.4 containing 0.1% Tween) containing 5% non-fat dry milk for at least 120 min at room temperature and exposed to the following primary antibodies overnight: Anti-γH2A.X (phospho S139), rabbit monoclonal Ab, Abcam, #ab81299, diluted 1:1000), anti-p53 rabbit Ab (#9282S; Cell Signaling Technologies, diluted 1:1000), anti-phospho-p53 (S15) rabbit Ab (#9284S; Cell Signaling Technologies, diluted 1:1000), anti-p38 MAPK rabbit Ab (#9212S; Cell Signaling Technologies, diluted 1:1000), anti-phospho-p38 MAPK (T180/Y182) (12F8) rabbit Ab (#4631S; Cell Signaling Technologies, diluted 1:1000), anti-Catalase rabbit mAb (#14097S, Cell Signaling Technologies, diluted (1:1000), anti-superoxide dismutase 1 (SOD-1) rabbit polyclonal IgG (sc-11407, Santa Cruz Biotechnology, diluted 1:5000), anti-superoxide dismutase 2 (SOD-2) rabbit polyclonal IgG (sc-30080, Santa Cruz Biotechnology, diluted 1:5000), anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH; MAB374, Millipore, Darmstadt, Germany, diluted (1:10000)).

Techniques: Western Blot, Phospho-proteomics, Control, Irradiation, Expressing, Marker, Gene Expression, Staining, Immunohistochemistry, Comparison, MANN-WHITNEY